• Taqman SNP genotyping can be ordered for readymade, user specified or custom assays. Large sample size is required to obtain an accurate allele discrimination plot. The samples for a study can be submitted in small batches and can be pooled and analyzed later.
• We accept whole blood samples and genomic DNA samples for the different type of assays. Please refer to the table for sample submission guidelines.
• Each sample should be accompanied by a requisition form.
• For DNA samples, quality will be assessed in the facility after sample reception and in case of samples not meeting QC resubmission may be requested.
• It is recommended that the DNA samples for genotyping be purified by column/magnetic bead methods.

*Large sample size is required for producing accurate allele discrimination plot

For further details on sample submission please contact uaeugenomics@uaeu.ac.ae

• Genotyping requisition form

Enquire: uaeugenomics@uaeu.ac.ae

Targeted region sequencing is an effective approach for studying selected regions of interest (e. g. gene panels) within the genome. A panel of probes or primers for target regions can be designed and used for enriching the regions of interests from whole genomic DNA.

Custom targeted sequencing is a cost-effective solution for sequencing a small number of genes with high coverage. Target definition, panel design and validation are specific for each panel and this service can be availed after consultation with our in-house NGS specialists.

RNA-seq or RNA sequencing is performed to analyze the sequence and quantity of RNA in a sample by NGS. This technique is useful for analyzing the whole transcriptome of an organism for gene expression profiling, RNA splicing/editing and differential gene expression analysis. RNA-seq workflows can be customized according to the research needs after consultation with in-house NGS specialists.

Metagenomics is a tool used for analyzing DNA acquired from environmental samples directly, eliminating the need for pure cultures of microorganisms. NGS sequencing permits parallel high-resolution sequencing of all the DNA fragments in a mixed sample of microrganisms.

The sequences can be aligned to a reference sequence database for taxonomical classification and determining the identity of the microbes in a sample. Clinical applications of metagenomics include diagnosis of infectious diseases, pathogen discovery, outbreak tracking and surveillance. Metagenomics analysis employs different approaches such as 16SRna sequencing and Shotgun sequencing based on your target community.

De novo sequencing is a derivative of Whole genome sequencing, for assembling genomes without any prior knowledge of the sequence. Sequence reads are assembled by contig assembly without alignment on to a previous reference sequence.

The coverage quality of de novo sequence data depends on the length and continuity of the contigs. It Provides useful information for mapping genomes of novel organisms or finishing genomes of known organisms, identifies structural variants and complex rearrangements, such as deletions, inversions, or translocations and Generates accurate reference sequences, even for complex or polyploid genomes.

The methylation of cytosines is a major epigenomic mechanism that modulates the primary genomic code. This leads to the formation of 5-methylcytosines at select sites of the genome. In methylation sequencing, both Whole-Genome Bisulfite Sequencing and Reduced Representation Bisulfite Sequencing are used in order to convert cytosine residues to uracil, leaving the 5-methylcytosine residues unaffected.

Therefore, DNA that has been treated with bisulfite retains only methylated cytosines. Thus, bisulfite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, this enables us to align the generated sequence onto a reference genome and determine the converted and non-converted basepairs and perform Epigenomic mapping.

ChIP-Seq, or ChIP-sequencing, is a technique that involved both chromatin immunoprecipitation technique the massive parallel sequencing capability of NGS. Chromatin immunoprecipitation lets us identify specific DNA sequences bound to proteins of interest. This process involves fixation of chromatin with formaldehyde through covalent linkages between DNA-binding proteins and DNA, cell lysis, Mechanical shearing of DNA, wherein specific DNA–protein complexes are isolated through immunoprecipitation with protein-specific antibodies.

Then, the isolate DNA is amplified by PCR. And the generated amplicons are sequenced using Next Generation Sequencing. ChIP-seq is vital and incredibly useful in epigenomic research. Whole Genome analysis of histone modifications, enables fundamental analysis the epigenetic state in a sequence or Sequences contributes to cell identity, development, lineage specification, and disease.